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dc.contributor.authorSimba Lavhelesani Dembe
dc.date.accessioned2013-03-26T09:55:59Z
dc.date.available2013-03-26T09:55:59Z
dc.date.created2013en
dc.date.issued2013-03-26T09:55:59Z
dc.identifier.urihttp://hdl.handle.net/123456789/1288
dc.description.abstractPheidole megacephala (Fabricius, 1793) is an ant species of the family Formicidae that includes at least ten subspecies. It is one of the three species that are found across the Soutpansberg Mountain. The mountain is characterised by a strong environmental gradient and raises the question whether the taxon includes cryptic species. The study was conducted along a north-south altitudinal transect. We hypothesized that P. megacephala found in this transect is one species. DNA barcoding was used to determine if there are any cryptic species within this species group. DNA extraction was conducted on the whole ant using the Qiagen extraction kit after which the DNA was viewed on a 1.75% agarose gel. The polymerase chain reaction was used for DNA amplification, followed by sequencing and phylogenetic analysis where the Neighbour joining, Maximum likelihood and Maximum. Sequence extraction and amplification was only successful for two specimens. Phylogenetic analysis suggests that the two specimens belong to different clades, and that P. megacephala species group along the mountain consist of cryptic species. These conclusions are however confounded by the small sample size and the possibility of misidentifications based on morphologyen
dc.description.statementofresponsibilityFoord S.H
dc.format.extent5301272 bytes
dc.format.mimetypeapplication/msword
dc.languageEnglishen
dc.language.isoen_US
dc.rightscopyrighten
dc.subjectCryptic speciesen
dc.subjectDNA barcodingen
dc.subjectPhylogeneticsen
dc.subjectLajumaen
dc.titleShould we lump or split? The case of the Pheidole megacephala species group along a strong environmental gradient in the Soutpansberg Mountains in the Limpopo Province, South Africa?en
dc.mdidentification.purposeBaseline ants data for altitudinal transect and factors that genetically determine if the P. megacephala species group found in the Soutpansberg consist of one or several species.en
dc.mdidentification.organizationnameCentre of Excellence for Invasion Biologyen
dc.mdidentification.deliverypointCentre for Invasion Biology University of Venda School of mathematics and natural science Department of Zoology Private BagX 5050 Thohoyandouen
dc.mdidentification.postalcode0950en
dc.mdidentification.phone0159628492en
dc.mdidentification.electronicmailaddressstefan.foord@univen.ac.zaen
dc.mddataidentification.languageEnglishen
dc.mdusage.usagedatetime2013-03-20
dc.mdlegalconstraints.accessconstraintscopyrighten
dc.lilineage.statementAll sites were georeferenced to 5m accuracy. Ant samples were submitted to the International Barcode of Life (IBOL) for sequencing The sequences were submitted to BLAST (Basic Local Alignment Search Tool) in NCBI (National Center for Biotechnology Information) to verify the species of the sequenced product.en
dc.dqcompletenessomission.valueunitPercentageen
dc.dqcompletenessomission.valueattributedata100en
dc.mdmaintenanceinformation.maintenanceandupdatefrequencyAnnuallyen
dc.mdmaintenanceinformation.dateofnextupdate2013-03-20
dc.mdfeaturecataloguedescription.cataloguedate2013-03-20
dc.mddistributor.distributorcontactUniversity of Vendaen
dc.mdformat.nameMS Officeen
dc.mdformat.versionMS Office 2010en
dc.exgeographicboundingbox.westboundlongitude29.4 1646°en
dc.exgeographicboundingbox.eastboundlongitude29.49005°en
dc.exgeographicboundingbox.northboundlattitude22.94472°en
dc.exgeographicboundingbox.southboundlattitude23.06423°en
dc.exverticalextent.minimumvalue800en
dc.exverticalextent.maximumvalue1747en
dc.exverticalextent.unitofmeasuremetersen
dc.cibprojectLONG-TERM COLLABORATIVE RESEARCHen


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