Karyotypic evolution in Malagasy flying foxes (Pteropodidae, Chiroptera) and their hipposiderid relatives as determined by comparative chromosome painting
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Pteropodidae and Hipposideridae are 2 of the 9 chiropteran families that occur on Madagascar. Despite major advancements in the systematic study of the island’s bat fauna, few karyotypic data exist for endemic species. We utilized G- and C-banding in combination with chromosome painting with Myotis myotis probes to establish a genome-wide homology among Malagasy species belonging to the families Pteropodidae ( Pteropus rufus 2n = 38; Rousettus madagascariensis , 2n = 36), Hipposideridae ( Hipposideros commersoni s.s., 2n =52), and a single South African representative of the Rhinolophidae ( Rhinolophus clivosus , 2n = 58). Painting probes of M . myotis detected 26, 28, 28, and 29 regions of homology in R . madagascariensis , P . rufus , H . commersoni s.s, and R . clivosus, respectively. Translocations, pericentric inversions, and heterochromatin additions were responsible for karyotypic differences amongst the Malagasy pteropodids. Comparative chromosome painting revealed a novel pericentric inversion on P . rufus chromosome 4. Chromosomal characters suggest a close evolutionary relationship between Rousettus and Pteropus. H. commersoni s.s. shared several chromosomal characters with extralimital congeners but did not exhibit 2 chromosomal synapomorphies proposed for Hipposideridae. This study provides further insight into the ancestral karyotypes of pteropodid and hipposiderid bats and corroborates certain molecular phylogenetic hypotheses.
- RESEARCH: Taylor, P