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dc.contributor.authorda Silva, J.M.
dc.contributor.authorFeldheim, K.A.
dc.contributor.authorMeasey, G.J.
dc.contributor.authorDoucette-Riise, S.
dc.contributor.authorDaniels, R.J.
dc.contributor.authorChauke, L.F.
dc.contributor.authorTolley, K.A.
dc.date.accessioned2017-08-04T12:01:37Z
dc.date.available2017-08-04T12:01:37Z
dc.date.issued2017
dc.identifier.citationDa Silva, J.M.; Feldheim, K.A.; Measey, G.J.; Doucette-Riise, S.; Daniels, R.J.; Chauke, L.F.; Tolley, K.A. (2017) Genetic diversity and differentiation of the Western Leopard Toad (Sclerophrys pantherina) based on mitochondrial and microsatellite markers. African Journal of Herpetology, 66(1): 25-38en
dc.identifier.issn2156-4575
dc.identifier.urihttp://hdl.handle.net/123456789/2299
dc.description.abstractIntraspecific genetic diversity provides the basis for evolutionary change and is therefore considered the most fundamental level of biodiversity. Mitochondrial DNA (mtDNA) and microsatellite loci are the markers most typically used in population-level studies; however, their patterns of genetic variation are not always congruent. This can result in different interpretations of the data, which can impact on management decisions, especially for threatened species. Consequently, in this study, we developed and analysed novel microsatellite markers for the Endangered Western Leopard Toad (WLT), Sclerophrys pantherina, and compared the results to previously published mtDNA data to compare the level of genetic diversity between the two molecular markers. The microsatellite evidence showed signs of a past bottleneck, yet relatively high levels of genetic diversity and low genetic differentiation between two sampling sites. In contrast, the mtDNA revealed moderate to low levels of diversity between sampling sites, and strong genetic differentiation. An explanation for the conflicting patterns may be that the current genetic signature, as depicted by the microsatellite data, is not yet reflected in the mitochondrial dataset; and, as such the data are depicting a timeline for genetic variation within the WLT. Both markers revealed important information about the two sampling sites, which can help inform conservation management of the species.en
dc.format.extent934460 bytes
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.publisherTaylor & Francisen
dc.subjectAfricaen
dc.subjectAmietophrynusen
dc.subjectBufonidaeen
dc.subjectconservationen
dc.subjectendangereden
dc.subjectgenetic variationen
dc.titleGenetic diversity and differentiation of the Western Leopard Toad (Sclerophrys pantherina) based on mitochondrial and microsatellite markersen
dc.typeArticleen


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